Journal: World Journal of Gastroenterology

Article Title: Huangqin decoction alleviates lipid metabolism disorders and insulin resistance in nonalcoholic fatty liver disease by triggering Sirt1/NF-κB pathway

doi: 10.3748/wjg.v29.i31.4744

Figure Lengend Snippet: Huangqin decoction activates the Sirt1/NF-Κb pathway in high-fat diet-induced nonalcoholic fatty liver disease rats. Data are presented as mean ± SD ( n = 3–4). a P < 0.01 vs the normal group, b P < 0.05 and c P < 0.01 vs the high-fat diet group. A: Expression levels of Sirt1, p-NF-κB/NF-κB, p-IRS-2/IRS-2, sterol regulatory element-binding protein (SREBP)-1c, fatty acid synthase, and cluster of differentiation 36; B-G: Semi-quantitative analysis of these proteins; H-J: Representative images of immunohistochemical staining of Sirt1 (H), NF-κB (I), and SREBP-1c (J) in liver sections; K-M: Quantitative analysis of the staining intensity; N: Representative images of immunofluorescent staining of Sirt1 and NF-κB in liver sections; O and P: Quantitative analysis of the fluorescence intensity. CD36: Cluster of differentiation 36; DAPI: 4',6-diamidino-2-phenylindole; FAS: Fatty acid synthase; GAPDH: Glyceraldehyde-3-phosphate dehydrogenase; HFD: High-fat diet; HQD, Huangqin decoction; SREBP: Sterol regulatory element-binding protein.

Article Snippet: A colorimetric 2-deoxydglucose (2DG) uptake assay kit and antibodies against IL-1β, sterol regulatory element-binding protein (SREBP)-1, fatty acid synthase (FAS), cluster of differentiation 36 (CD36), Sirt1, NF-κB, p-NF-κB, IRS-2, p-IRS-2, and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) were purchased from Abcam (Cambridge, MA, United Kingdom).

Techniques: Expressing, Binding Assay, Immunohistochemistry, Staining, Fluorescence

Journal: World Journal of Gastroenterology

Article Title: Huangqin decoction alleviates lipid metabolism disorders and insulin resistance in nonalcoholic fatty liver disease by triggering Sirt1/NF-κB pathway

doi: 10.3748/wjg.v29.i31.4744

Figure Lengend Snippet: Huangqin decoction activates the Sirt1/NF-κB pathway in palmitic acid-induced HepG2 cells. Data are presented as mean ± SD of at least three independent experiments. a P < 0.01 vs the control group, b P < 0.05 and c P < 0.01 vs the palmitic acid group. A: Expression levels of Sirt1, p-NF-κB/NF-κB, p-IRS-2/IRS-2, sterol regulatory element-binding protein 1, fatty acid synthase, and cluster of differentiation 36 in HepG2 cells; B–G: Semi-quantitative analysis of these proteins; H: Representative images of immunofluorescent staining of Sirt1 and NF-κB in HepG2 cells. CD36: Cluster of differentiation 36; DAPI: 4',6-diamidino-2-phenylindole; FAS: Fatty acid synthase; GAPDH: Glyceraldehyde-3-phosphate dehydrogenase; HQD, Huangqin decoction; PA: Palmitic acid; SREBP: Sterol regulatory element-binding protein.

Article Snippet: A colorimetric 2-deoxydglucose (2DG) uptake assay kit and antibodies against IL-1β, sterol regulatory element-binding protein (SREBP)-1, fatty acid synthase (FAS), cluster of differentiation 36 (CD36), Sirt1, NF-κB, p-NF-κB, IRS-2, p-IRS-2, and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) were purchased from Abcam (Cambridge, MA, United Kingdom).

Techniques: Expressing, Binding Assay, Staining

Journal: World Journal of Gastroenterology

Article Title: Huangqin decoction alleviates lipid metabolism disorders and insulin resistance in nonalcoholic fatty liver disease by triggering Sirt1/NF-κB pathway

doi: 10.3748/wjg.v29.i31.4744

Figure Lengend Snippet: Huangqin decoction protects hepatocytes by triggering Sirt1/NF-κB pathway in palmitic acid-induced HepG2 cells. Data are presented as mean ± SD of at least three independent experiments. a P < 0.01 vs the control group, b P < 0.05 and c P < 0.01 vs the palmitic acid group, d P < 0.05 and e P < 0.01 vs the Huangqin decoction group. A and B: Levels of triacylglycerol (A) and total cholesterol (B) in HepG2 cells co-cultured with or without EX-527; C-E: Levels of interleukin (IL)-6, IL-1β, and tumor necrosis factor-α in HepG2 cells co-cultured with or without EX-527; F: Level of glucose uptake in HepG2 cells co-cultured with or without EX-527; G: Representative micrographs of Oil Red O staining of HepG2 cells co-cultured with or without EX-527; H: Expression levels of Sirt1, p-NF-κB/NF-κB, p-IRS-2/IRS-2, sterol regulatory element-binding protein-1c, fatty acid synthase, and cluster of differentiation 36 in HepG2 cells co-cultured with or without EX-527; I-N: Semi-quantitative analysis of these proteins; O: Representative images of immunofluorescent staining of Sirt1 and NF-κB in HepG2 cells co-cultured with or without EX-527. 2DG: 2-deoxydglucose; CD36: Cluster of differentiation 36; DAPI: 4',6-diamidino-2-phenylindole; FAS: Fatty acid synthase; GAPDH: Glyceraldehyde-3-phosphate dehydrogenase; HQD: Huangqin decoction; IL: Interleukin; PA: Palmitic acid; SREBP: Sterol regulatory element-binding protein; TC: Total cholesterol; TG: Triacylglycerol; TNF: Tumor necrosis factor.

Article Snippet: A colorimetric 2-deoxydglucose (2DG) uptake assay kit and antibodies against IL-1β, sterol regulatory element-binding protein (SREBP)-1, fatty acid synthase (FAS), cluster of differentiation 36 (CD36), Sirt1, NF-κB, p-NF-κB, IRS-2, p-IRS-2, and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) were purchased from Abcam (Cambridge, MA, United Kingdom).

Techniques: Cell Culture, Staining, Expressing, Binding Assay